Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc02088g Click here for additional data file.

Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells.

We present new fluorophore-conjugates for dual-color photoactivation and super-resolution imaging inside live mammalian cells. These custom-designed, photo-caged Q-rhodamines and fluoresceins are cell-permeable, bright and localize specifically to intracellular targets. We utilized established orthogonal protein labeling strategies to precisely attach the photoactivatable fluorophores to protei...

Dynamic multicolor protein labeling in living cells† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01364g Click here for additional data file. Click here for additional data file. Click here for additional data file.

Protein-based fluorescent nanoparticles for super-resolution STED imaging of live cells† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc04664a Click here for additional data file.

Institute of Applied Physics, Karlsruhe Instit Germany Institute of Nanotechnology, Karlsruhe Eggenstein-Leopoldshafen, Germany Center for Nano Energy Materials, Schoo Northwestern Polytechnical University, 71 nwpu.edu.cn Laboratory of Electron Microscopy, Karlsru Karlsruhe, Germany Institute of Toxicology and Genetics, Karlsr Eggenstein-Leopoldshafen, Germany Department of Physics, University ...

iPAINT: a general approach tailored to image the topology of interfaces with nanometer resolution† †Electronic supplementary information (ESI) available: Fig. S1–S8. See DOI: 10.1039/c6nr00445h Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.

Understanding interfacial phenomena in soft materials such as wetting, colloidal stability, coalescence, and friction warrants non-invasive imaging with nanometer resolution. Super-resolution microscopy has emerged as an attractive method to visualize nanostructures labeled covalently with fluorescent tags, but this is not amenable to all interfaces. Inspired by PAINT we developed a simple and ...

Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c4ra01027b Click here for additional data file.

We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.